Experimental instruments

Tube, Micropipettor, Vortex mixer, Low-speed centrifuge, 37℃ thermostat water bath (gas bath), Spectrophotometer (420 nm)

Application

This kit can be used for detection of Glutathione Reductase Activation Coefficient in samples, such as animal whole blood, serum (plasma), tissues etc.

Determination significance

Ribofavin binds zymoprotein into all kinds of flavoprotein in the form of FDA in vivo. It participates in redox process as electron transfer system. When ribofavin is deficient, it will produce a range of symptoms on skin, mucosa, ocular region etc. In order to find ribofavin deficiency in early stage and take control measures in time, it is necessary to evaluate the nutritional status of ribofavin.

When ribofavin is deficient, Glutathione Reductase Activation Coefficient increases rapidly; and it decreases to normal after replenishing ribofavin. So Glutathione Reductase Activation Coefficient (GRAC) is an accurate indicator of overall ribofavin level in vivo when affected with chronic ribofavin deficiency. Using GRAC value to evaluate the nutritional status of ribofavin has advantages such as sensitivity, stability, accuracy, microscale, and reflection of internal metabolic status. 

Operation steps

Mix and stand for 5 min. Measure the OD values of each tube at 420 nm with 0.5 cm diameter cuvette and set to zero with double-distilled water.