With the coenzyme as hydrogen donor, GSSG can be reduced to GSH under the catalysis of GR. Then the GSH content increased and NADPH decreased. The decrease of NADPH absorbance can be measured at 340 nm. The activity of GR can be calculated by detecting the change of NADPH.

1. Just test one sample for each time.

2. Temperature has a great influence on the reaction system. Preheat the cuvette with at 37℃ when measuring the absorbance.

3. The detection procedure should be operated quickly. The operation steps should be operated carefully and avoid pollution and splash. The time must be recorded accurately.

1.    Pre-heat cuvette in incubator at 37℃ for 5 min.

2.    Set the UV spectrophotometer at 340 nm wavelength, prepare a couple of 1 cm diameter quartz cuvette, one is used for sample detection, another is used for setting to zero with double-distilled water.

3.    Add 65 μL of sample into the tube, then add 3120 μL of working solution, mix immediately and record the time.

4.    Mix fully, measure the absorbance at 340 nm at 30 second (A1) and 180 second (A2), respectively. △A=A1-A2.