상품 정보
상품 기본설명
상품 상세설명
General information
Detection significance
Detection principle
The key point
The reagent 8 has a pungent odor. Please operate in the fume hood.
Operation procedures
Operation steps
1. Detection of T-GSH
1) The dilution of standards
Dilute 20 μmol/L Standard solution with reagent 3 working solution to a serial concentration. The recommended dilution gradient is as follows: 15, 10, 8, 5, 2, 1, 0.5, 0 μmol/L.
2) The Operation table
| Standard tube | Sample tube |
Standard solution with different concentration (μL) | 10 |
|
Sample (μL) |
| 10 |
Reactive working solution (μL) | 150 | 150 |
Mix fully, stand for 5 min at room temperature (25℃) | ||
Reagent 9 working solution (μL) | 50 | 50 |
Mix fully for 5 s with Microplate reader, then incubate at room temperature (25℃) for 25 min and measure the OD value of each well at 412 nm. | ||
2. Detection of GSSG
1) The pretreatment of standard:
Add 20 μL of reagent 7 working solution to 100 μL of the standard solution with different concentration (15, 10, 8, 5, 2, 1, 0.5, 0 μmol/L), mix fully with a vortex mixer, then take 100 μL od liquid to 0.5 mL EP tube and add 4 μL of reagent 8 working solution, mix fully with a vortex mixer immediately, react at 25℃ for an hour.
2) Remove the GSH of samples
Add 20 μL of reagent 7 working solution to 100 μL of pretreated samples , mix fully with a vortex mixer, then take 100 μL od liquid to 0.5 mL EP tube and add 4 μL of reagent 8 working solution, mix fully with a vortex mixer immediately, react at 25℃ for an hour.
3) The Operation table
| Standard tube | Sample tube |
Standard solution with different concentration (μL) | 10 |
|
Sample (μL) |
| 10 |
Reactive working solution (μL) | 150 | 150 |
Mix fully, stand for 5 min at room temperature (25℃) | ||
Reagent 9 working solution (μL) | 50 | 50 |
Mix fully for 5 s with Microplate reader, then incubate at room temperature (25℃) for 25 min and measure the OD value of each well at 412 nm. | ||
Performance characteristics
Technical parameter
| Detection range | 0.36-30 μmol/L | Average inter-assay CV | 3.9% |
| Sensitivity | 0.36 μmol/L | Average intra-assay CV | 0.6% |
| Average recovery rate | 97% |
상품문의
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상품문의가 없습니다.
반품/교환정보
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합니다.)
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