Application

This kit can be used to measure direct bilirubin (D-BIL) content in serum or plasma.

Detection significance

High total bilirubin in serum (plasma) is common in hepatitis, extrahepatic biliary obstruction, hemolytic disease, neonatal jaundice, etc..

Detection principle

Direct bilirubin is oxidized under vanadonic acid to form biliverdin. The decrease of absorbance at 450nm is proportional to the content of direct bilirubin..

Experimental material

Microplate (96 wells), High-precision transferpettor, Microplate Reader (450 nm)

Operation steps (Microplate reader)

The reference range

Healthy human serum: 0-6.84 µmol/L (0-0.04 mg/dL) (This is for reference only.)

Limitations

1. Interferential substance: Hemoglobin≤0.2 g/L,Vitamin C≤30 mg/dL, Glycerin trilaurate≤2000 mg/dL, have no interference to the result.

2. direct bilirubin is just one of the indicators of the experiment. It is also necessary to make a comprehensive judgment according to the physical signs of the sample and other experimental indexs.

Product performance index

1. The OD value of blank is not more than 0.05..

2. Linear range : 1.0 - 410 µmol/L (a: R2≥0.995. b: 1.0-150 µmol/L, deviation from linearity is less than 20.0 µmol/L. c: 150-410 µmol/L, deviation from linearity is less than 10% ).

3. Precision: the CV% is less than 10%.

4. Recovery: 100±20%.

5. Sensitivity: When the concentration of sample is 50 µmol/L, the difference of absorbance (△A) is more than 0.04.

Notes

1. The working solution is corrosive, it should be avoided to contact with skin and clothing. Wash immediately with plenty of water if contact it carelessly.

2. Instructions should be followed strictly.

3. All the samples and waste material should be treated as infective material according to the relevant rules of biosafety.

4. Bilirubin can be easily decomposed by light and it should be avoid light when measure.