Overview

The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is Omega Bio-tek’s HiBind® matrix that specifically, but reversibly, binds DNA or RNA under optimal conditions, while allowing proteins and other contaminants to be removed. The nucleic acids bound to the HiBind® matrix are easily eluted with deionized water or low salt buffer. DNA and RNA are suitable for many downstream applications.

Omega Bio-tek’s E.Z.N.A.® M13 DNA Kits are designed to purify up to 10 µg of single stranded DNA from up to 3 mL of phage supernatant. Yields of single stranded DNA obtained using E.Z.N.A.® M13 DNA Kits are around 3-10 µg and reproducible when the isolations are performed from the same culture. 

Specifications

For Research Use Only. Not for use in diagnostic procedures. 

Product Data

Figure 1 Title

Figure 1.  DNA purified using the E.Z.N.A.® M13 DNA Kit. DNA was isolated from 4 1 mL M13 supernatant cultures grown 6 hours in E. coli culture using the E.Z.N.A.® M13 DNA Kit and eluted in 50 µL TE buffer. M13 ssDNA (5% of total purified DNA) was analyzed on a 1.0% agarose gel. Lanes 1-4: M13 ssDNA, M: 1 kb DNA formaldehyde agarose gel.. 

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