Overview

By adapting Hi-Bind technology with 96-well plates, the E-Z 96® Tissue DNA Kit provides a high-throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridization techniques.

• Rapid – Isolation of DNA from 96 samples in less than 1 hour
• Reliable – Reproducible DNA purification from variety of samples
• Safe – No phenol/chloroform extractions
• Purified DNA suitable for most downstream application

Specifications

For Research Use Only. Not for use in diagnostic procedures.

Product Data

Genomic DNA purified from various chicken tissues using E-Z 96® Tissue DNA Kit is of good quality.

Figure 1. Genomic DNA purified using the E-Z 96® Tissue DNA Kit. Genomic DNA was extracted from chicken tissue: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. The 5% of each eluate genomic DNA was analyzed on a 0.8% agarose gel to demonstrate the yield and quality of the DNA. 

Genomic DNA purified from various chicken tissues using E-Z 96® Tissue DNA Kit performed successful PCR amplification.

Figure 2. Amplification of genomic DNA isolated from chicken tissue samples using the E-Z 96® Tissue DNA Kit. DNA was isolated from chicken tissue in duplicate: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. A total of 2 µL of each eluate was amplified with a PCR mastermix and NS gene primers for a 750 bp product to determine whether inhibitors were present in the eluted DNA. Each lane contains 10% of PCR product separated on a 1% agarose gel. 

Genomic DNA purified from various chicken tissues using E-Z 96® Tissue DNA Kit has excellent yield and quality.

Figure 3. Genomic DNA purified using the E-Z 96® Tissue DNA Kit. Genomic DNA was extracted from chicken tissue: sample 1 from spleen, sample 2 from lung, sample 3 from muscle, and sample 4 from liver. The 5% of each eluate genomic DNA was analyzed on a 0.8% agarose gel to demonstrate the yield and quality of the DNA. 

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