Oxford Medium Base / Code: REF222530
Specifications | |||||
---|---|---|---|---|---|
Formula | Pancreatic Digest of Casein | 8.9g | |||
Proteose Peptone No. 3 | 4.4g | ||||
Yeast Extract | 4.4g | ||||
Tryptic Digest of Beef Heart | 2.7g | ||||
Starch | 0.9g | ||||
Sodium Chloride | 4.4g | ||||
Esculin | 1.0g | ||||
Ferric Ammonium Citrate | 0.5g | ||||
Lithium Chloride | 15.0g | ||||
Agar | 15.3g | ||||
Directions | 1. Suspend 57.5 g of the powder in 1 L of purified water. Mix thoroughly. Difco™ or BBL™ Buffered Peptone Water – 20 g; Difco™ Buffered Peptone Casein Water – 20 g. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 10 minutes. Cool to 45-50°C. 4. To prepare Oxford Medium: Dissolve acriflavine (5.0 mg), cefotetan (2.0 mg), colistin sulfate (20.0 mg), cycloheximide (400.0 mg) and fosfomycin (10.0 mg) in 5 mL reagant grade ethanol and 5 mL purified water. Filter sterilize supplement before use. Add 10 mL of antimicrobic supplement to 1 L of molten Oxford Medium Base (45-50°C). Mix thoroughly. To prepare Modified Oxford Medium: Aseptically rehydrate one vial of Modified Oxford Antimicrobic Supplement with 10 mL of sterile purified water. Rotate in an end-over-end motion to dissolve the contents completely. Add 10 mL of rehydrated Modified Oxford Antimicrobic Supplement to 1 L of Oxford Medium Base (45-50°C). Mix thoroughly. 5. Test samples of the finished product for performance using stable, typical control cultures. | ||||
*Adjusted and/or supplemented as required to meet performance criteria. |
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