Magnetic Beads Genomic DNA Extraction Kit (Plant) MP048/MP096

The Magnetic Beads Genomic DNA Extraction Kit Plant was designed specifically for efficient genomic DNA purification from a variety of plant species. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Genomic DNA Extraction Kit Plant can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and other downstream applications. 

Advantages (Cat. # MP048, MP096)

• High Yield: 1-20 µg of Genomic DNA
• High Quality DNA: A260/A280 = 1.8-1.9
• Magnetic Bead Concentration: 50 mg/ml
• Magnetic Bead Size: ~ 5 µm
• Easily adapted to automated magnetic bead separation instruments and workstations
• Sample: 10-50 mg of fresh or frozen plant tissue
• Manual or automated DNA isolation
• Operation time: within 30 minutes (manual) 
• Storage: dry at room temperature (15-25ºC)

• MP1 Buffer
• MP2 Buffer
• RNase A
• MW1 Buffer
• MW2 Buffer
• MP Magnetic Beads
• Elution Buffer

	Figure 1. Genomic DNA was extracted using the Magnetic Beads Genomic DNA Extraction Kit Plant. 5 µl of purified genomic DNA from 2 different plant leaves was analyzed on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder Lane 1-3 = Benthamiana nicotiana leaf Lane 4-6 = Tomato leaf
Plant Species Sample DNA Conc. 260/280 Yield Benthamiana nicotiana 50 mg leaf 30 ng/µl 1.7-1.8 3 µg Tomato 50 mg leaf 65 ng/µl 1.7-1.8 6.5 µg Table 2. Yield of purified DNA from 50 mg fresh leaf samples.
	Figure 2. Genomic DNA was extracted using the Magnetic Beads Genomic DNA Extraction Kit Plant. 2 µl of purified genomic DNA from 3 different plant leaves was analyzed on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder Lane 1-2 = Arabidopsis leaf Lane 3-4 = lemon leaf Lane 5-6 = coffee leaf
Plant Species Sample DNA Conc. 260/280 Yield Arabidopsis 50 mg leaf 20 ng/µl 1.8-1.9 2 µg Lemon 50 mg leaf 150 ng/µl 1.8-1.9 15 µg Coffee 50 mg leaf 90 ng/µl 1.7-1.8 9 µg Table 2. Yield of purified DNA from 50 mg fresh leaf samples.