The Magnetic Beads Genomic DNA Extraction Kit Bacteria was designed specifically for efficient genomic DNA purification from Gram (-) negative and Gram (+) positve bacteria. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and a variety of other downstream applications. 

Advantages (Cat. # ME048, ME096)

• High Yield: >26 µg of Genomic DNA
• High Quality DNA: A260/A280 = 1.8-2.0
• Magnetic Bead Concentration: 50 mg/ml
• Magnetic Bead Size: ~ 5 µm
• Easily adapted to automated magnetic bead separation instruments and workstations
• Sample: 2 x 109 of bacteria cells (Gram + and Gram -)
• Manual or automated DNA isolation
• Operation time: within 30 minutes (manual) 
• Storage: dry at room temperature (15-25ºC), Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods

• ME1 Buffer
• ME2 Buffer
• ME3 Buffer
• ME4 Buffer
• Lysozyme
• Proteinase K
• MW1 Buffer
• MW2 Buffer
• ME Magnetic Beads
• Elution Buffer

	Figure 1. Genomic DNA from 1 x 109 cells of Escherichia coli and from  1 x 109 cells of Bacillus subtilis was extracted in triplicate using the Magnetic Beads Genomic DNA Extraction Kit Bacteria. 5 μl aliquots of extracted DNA product from a 100 μl eluate were analyzed on a 0.8% agarose gel. Lane 1-3: Escherichia coli M = Geneaid 1 Kb DNA Ladder Lane 4-6: Bacillus subtilis
Test Sample Bacteria Strain 260/280 260/230 Yield 1 Escherichia coli 2.06 1.95 24.48 µg 2 Escherichia coli 2.06 1.90 22.49 µg 3 Escherichia coli 2.07 2.02 26.47 µg 4 Bacillus subtilis 2.06 2.28 26.27 µg 5 Bacillus subtilis 2.06 2.24 22.20 µg 6 Bacillus subtilis 2.05 2.25 26.46 µg Table 1. Yield of Genomic DNA from 1 x 109 cells of Escherichia coli and from  1 x 109 cells of Bacillus subtilis extracted in triplicate using the Magnetic Beads Genomic DNA Extraction Kit Bacteria.
	Figure 2. Extracted DNA products were used as a DNA template for amplifying 16S ribosomal RNA gene. 5 μl aliquots of PCR product were loaded in each well. A 1.5 kb 16S ribosomal gene fragment was successfully amplified from each DNA product. Lane 1-3: Escherichia coli M = Geneaid 1 Kb DNA Ladder Lane 4-6: Bacillus subtilis