Magnetic Beads Genomic DNA Extraction Kit (Blood) MB048/MB096

The Magnetic Beads Genomic DNA Extraction Kit Blood was designed specifically for efficient genomic DNA purification from blood and buffy coat. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Genomic DNA Extraction Kit Blood can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and Southern Blotting. 

Advantages (Cat. # MB048, MB096)

• High Yield: up to 10 µg of Genomic DNA
• High Quality DNA: A260/A280 = 1.8-2.0
• Magnetic Bead Concentration: 50 mg/ml
• Magnetic Bead Size: ~ 5 µm
• Easily adapted to automated magnetic bead separation instruments and workstations
• Sample: 100 to 400 µl of whole blood and buffy coat (5 x 106 WBC)
• Manual or automated DNA isolation
• Operation time: within 20 minutes (manual) 
• Storage: dry at room temperature (15-25ºC), Protease should be stored dry at 2-8ºC for extended periods

• MB1 Buffer
• MB2 Buffer
• Protease
• MW1 Buffer
• MW2 Buffer
• MB Magnetic Beads
• Elution Buffer

	Figure 1. 5 µl aliquots of extracted DNA from a 100 µl eluate were analyzed on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder, Lane 1-3 = 100 µl blood sample, Lane 4-6 = 200 µl blood sample, Lane 7-9 = 300 µl blood sample, Lane 10-12 = 400 µl blood sample.
Sample Volume # of WBC Yield 260/280 260/230 Whole Blood 100 µl 5.0 x 105 2.5-3.0 µg >1.8 >1.2 200 µl 1.0 x 106 5.5-6.0 µg >1.8 >1.6 300 µl 1.5 x 106 7.5-8.0 µg >1.8 >1.7 400 µl 2.0 x 106 9.5-10.0 µg >1.8 >1.8 Table 1. Yield of purified DNA from 100-400 µl whole blood samples.
	Figure 2. Extracted DNA products were used as a DNA template for amplifying partial human ACTB gene. 3 μl aliquots of PCR product were loaded in each well. A 250 bp ACTB gene fragment was successfully amplified from each DNA product. M1-2: Geneaid 1 Kb DNA Ladder, Lane 1-3: DNA from 100 µl blood sample, Lane 4-6: DNA from 200 µl blood sample, Lane 7-9: DNA from 300 µl blood sample, Lane 10-12: DNA from 400 µl blood sample