Magnetic Beads Virus DNA/RNA Extraction Kit MV048/MV096

The Magnetic Beads Virus DNA/RNA Extraction Kit was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. Viral DNA/RNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Viral DNA/RNA Kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA/RNA can be used in qPCR and qRT-PCR assays. 

Advantages (Cat. # MV048, MV096)

• High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number!
• Easily adapted to automated magnetic bead separation instruments and workstations
• Magnetic Bead Concentration: 50 mg/ml
• Magnetic Bead Size: ~ 5 µm
• Sample: up to 200 µl of virus samples (plasma, serum, body fluid or the supernatant of viral infected cell cultures) 
• Manual or automated DNA/RNA isolation
• Operation time: within 30 minutes (manual) 
• Storage: dry at room temperature (15-25ºC)

• MV1 Buffer
• MV2 Buffer
• MV3 Buffer
• MV4 Buffer
• RNase-free Water
• MV Magnetic Beads
• Carrier RNA

Figure 1. Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the Geneaid Magnetic Beads Virus DNA/RNA Extraction Kit (2 replications of each copy number). The purified RNA was eluted with 50 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 5 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV.