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96 Well Bacteria DNA Kit (96GBB04, 96GBB10)
The Presto™ 96 Well gDNA Bacteria Kit is optimized for high-throughput genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with Lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Proteinase K and chaotropic salt are used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the binding plate. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.
Advantages (Cat. # 96GBB04, 96GBB10)
- High-throughput bacteria DNA extraction
- High Yield: up to 5 μg of genomic DNA per well
- High Purity gDNA: A260/A280 >1.9
- DNA Size: 20-40 kb
- Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 5 x 108)
- Convenient: Includes Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
- 96 Well Binding Plates: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
- Elution volume: 100-200 µl
- Storage: dry at room temperature (15-25°C), Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods
- Proteinase K
- Gram+ Buffer
- GT Buffer
- GB Buffer
- W1 Buffer
- Wash Buffer
- Lysozyme
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- Presto™ gDNA 96 Well Binding Plates
- Microtubes (Racked)
- Microtubes (8-strip)
- Caps for Microtubes (8-strip)
- 96 Deep Well Plates
- Adhesive Film
Quality Control
 | Figure 1. Genomic DNA was extracted from bacteria cells using the Presto™ 96 Well gDNA Bacteria Kit. The purified genomic DNA was eluted in 100 µl of Elution Buffer and 10 µl aliquots of the final sample (chosen from 6 random wells) were analyzed by electrophoresis on a 1% agarose gel. M = Geneaid™ 1 Kb DNA Ladder 1-3: 5 x 108 Escherichia coli cells, 4-6: 5 x 108 Bacillus subtilis cells
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Specifications | ||||||||
| Cat. # | GS100 GS300 | GB100 GB300 | GT050 GT100 GT300 | GP100 GPM010 GPM025 | GBB100/101 GBB300/301 | GBY100 GBY300 | GMB100 GMB300 | GSK100 GSK300 |
Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Reagent System | Spin Column | Spin Column |
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg (Mini) 500 µg (Maxi) | 50 µg | N/A | 50 µg | 50 µg |
| Sample | 200 µl blood | 300 µl blood | 30 mg animal tissue | Mini: 100 mg plant tissue | 1x109 bacterial cells | 2x108 yeast cells | 1-100 µl blood | buccal swabs |
| 25 mg animal tissue | 200 µl buffy coat | Maxi: 1 g plant tissue | 200 µl blood | FTA® Cards | ||||
| 1x107 cultured cells | 1x107 cultured cells | 1 ml urine, plasma | 1 ml urine | |||||
hair | 2x108 yeast/fungus | 1-100 µl saliva | ||||||
| Micropestle | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A |
| Filter Column | N/A | N/A | N/A | Yes | N/A | N/A | N/A | Yes |
| Enzyme | Proteinase K | N/A | Proteinase K | N/A | Lysozyme Proteinase K | Lyticase or Zymolase | Proteinase K | Proteinase K |
Typical Yield | 5 µg from 200 µl blood | 4-10 µg from 300 µl blood | 10-40 µg from 20 mg tissue | 3-5 µg from 100 mg Arabidopsis leaf (Mini) | 37 µg from 1x109 E. coli | 10-15 µg from 2x108 S. cerevisiae cells | 300 ng from 6 mm blood spots | 2 µg per swab |
10-40 µg from 20 mg tissue | 80 µg from 500 mg Lemon leaf (Maxi) | 10-15 µg from 1x109 Bacillus subtilis | ||||||
| Elution Volume | 30-100 µl | 30-200 µl | 30-200 µl | 30-200 µl (Mini) 1 ml (Maxi) | 30-200 µl | 50-100 µl | 30-100 µl | 50-100 µl |
| Operation Time | <20 minutes | <25 minutes | 30 minutes | <30 minutes | <30 minutes | <40 minutes | <20 minutes | <20 minutes |
Product Name | RXNS | Catalogue Number |
100, 300 | GS100/300 | |
100, 300 | GB100/300 | |
10, 25 | GPM010/25 | |
10, 25 | GDM10/25 | |
50, 100, 300 | GT050/100/300 | |
100 | GP100 | |
100 | GMB100/300 | |
100 | GSK100/300 | |
100, 300 | GBY100/300 | |
100, 300 | GBB100/101/300/301 | |
4 x 96, 10 x 96 | 96GBP04/10 | |
Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 |
4 x 96, 10 x 96 | 96GBB04/10 |
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