상품 정보
상품 상세설명
The Presto™ 96 Well Plant Genomic DNA Extraction Kit is designed for high-throughput purification of total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from various plant species. Homogenized samples are treated with RNase A then centrifuged to remove cell debris and salt precipitates. In the presence of a binding buffer, genomic DNA in the lysate binds to the glass fiber matrix of the Presto™ 96 Well gDNA Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 60 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.
Advantages (Cat. # 96GPP04, 96GPP10)
- Purify 96 wells of pure genomic DNA from a variety of plant species in 60 minutes!
- Sample Volume: 100 mg of fresh plant tissue and 25 mg of dried plant tissue per well
- Yield: up to 30 μg of genomic DNA from 100 mg of fresh plant tissue per well
- Format: Presto™ 96 Well gDNA Binding Plates
- Elution Volume: 200~400 µl
- Centrifuge or vacuum manifold protocol
- Storage: dry at room temperature (15-25ºC)
Applications
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
Components
- GP1 Buffer and GPX1 Buffer
- GP2 Buffer
- GP3 Buffer
- RNase A
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- Presto™ gDNA 96 Well Binding Plates
- 96 Deep Well Plates
- Microtubes (Racked)
- Microtubes (8-strip)
- Caps for Microtubes (8-strip)
Quality Control
 | Figure 1. Genomic DNA was extracted from 50 mg of fresh corn leaf samples using the Presto™ 96 Well Plant Genomic DNA Extraction Kit. The purified genomic DNA was eluted in 200 µl of Elution Buffer and 15 µl aliquots of the final sample (chosen from 3 random wells) were analyzed by electrophoresis on a 1% agarose gel. M = Geneaid™ 1 Kb DNA Ladder
|
Product Name | RXNS | Catalogue Number |
100, 300 | GS100/300 | |
100, 300 | GB100/300 | |
10, 25 | GPM010/25 | |
10, 25 | GDM10/25 | |
50, 100, 300 | GT050/100/300 | |
100 | GP100 | |
100 | GMB100/300 | |
100 | GSK100/300 | |
100, 300 | GBYB100/300 | |
100, 300 | GBB100/101/300/301 | |
4 x 96, 10 x 96 | 96GBP04/10 | |
Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 |
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