RNA Cleanup Kit (PR050, PR100)

The RNA Cleanup Kit uses a simple and efficient spin column procedure to purify total RNA stored in RNase-free water, elution buffer or TE Buffer following extraction using acid-guanidinium-phenol-chlorofom based methods such as TRIzol® Reagent and GENEzol™ Reagent. Contaminants such as RNases, DNA and residual phenol are effectively removed using a simple 4 step procedure. The high-quality, total RNA is eluted in RNase-free Water or TE (RNase-free) and is ready for use in a variety of sensitive downstream applications. 

Advantages (Cat. # PR050, PR100)

• Purify up to 50 µg of total RNA in 10 minutes!
• Compatibility: purify RNA stored in RNase-free water, elution buffer or TE buffer following RNA extraction using GENEzol™, TRI-Reagent®, TRIzol®, RNAzol®, QIAzol® etc.
• Sample Volume: up to 100 μl of RNA products
• Membrane: glass fiber membrane optimized for total RNA extraction
• Spin Columns: individually packaged RNA spin columns, certified RNase and DNase-free
• Elution Volume: 20-50 μl
• Recovery: up to 80% of high-quality RNA (A260/A280 = 1.9-2.0)
• Storage: room temperature (15-25ºC)

• RNA Pure Buffer
• Wash Buffer
• RNase-free Water
• PR Columns
• 2 ml Collection Tubes

Figure 1. Total RNA was extracted using Supplier I Tri Reagent. An Escherichia coli (1×109) culture (OD600=1.3, 1 ml) was harvested by centrifugation at 16,000 x g for 1 minute. 10 µl from a 50 µl eluate of extracted RNA was analyzed by electrophoresis on a 0.8% agarose gel. Genomic DNA and other contaminants remain in the final product. M = Geneaid 1 Kb DNA Ladder Test RNA Yield 260/280 260/230 1 21.3 µg 1.67 0.72 2 20.2 µg 1.60 0.84 3 22.4 µg 1.63 1.07

Figure 2. Total RNA was extracted using Supplier I Tri Reagent then purified using the RNA Cleanup Kit. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel. Genomic DNA and other contaminants are removed. M = Geneaid 1 Kb DNA Ladder Test RNA Conc. 260/280 260/230 Yield Recovery 1 335.7 µg/ml 1.97 2.44 16.8 µg 78.9% 2 287.2 µg/ml 1.93 2.42 14.4 µg 71.3% 3 330.9 µg/ml 1.96 2.43 16.5 µg 73.7%