TriRNA Pure Kit (TRP050/TRP100/TRP200)

The TriRNA Pure Kit is a spin column system for purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in TRIzol® Reagent, GENEzol™ Reagent or other phenol, guanidine isothiocyanate reagents. Chloroform phase separation or isopropanol RNA precipitation is not required. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications. 

Advantages (Cat. # TRP050, TRP100, TRP200)

• Purify total RNA in 10 minutes!
• No chlorophorm phase separation
• No isopropanol RNA precipitation
• No phenol carryover
• Compatibility: homogenize samples with GENEzol™, TRI-Reagent®, TRIzol®, RNAzol®, QIAzol® etc.
• Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma), up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109 bacterial cells, 20-50 mg of plant tissue
• A cost effective phenol, guanidine isothiocyanate solution plus spin column system
• High quality RNA: A260/A280 >1.8, A260/A230 >1.8
• Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water
• Membrane: glass fiber membrane optimized for total RNA extraction
• Spin Columns: individually packaged RNA spin columns, certified RNase and DNase-free
• Storage: dry at room temperature (15-25ºC)

• Pre-Wash Buffer
• Wash Buffer
• RNase-free Water
• RB Columns
• 2 ml Collection Tubes

Figure 1. RNA was purified using the TriRNA Pure Kit in parallel to the similar product from competitor Z. 5x105 HeLa cells were homogenized using GENEzol™ Reagent and competitor Z tri reagent. RNA was then purified using the corresponing kits spin column procedure. 10 µl from a 50 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel. Test RNA Conc. 260/280 260/230 Yield 1. Z 162.5 ng/µl 2.00 2.07 8.1 µg 2. Z 160.7 ng/µl 2.03 2.07 8.0 µg 3. Geneaid 164.0 ng/µl 2.00 2.07 8.2 µg 4. Geneaid 161.6 ng/µl 2.03 2.06 8.0 µg Table 1. Total RNA purified using the TriRNA Pure Kit and competitor Z.

Test RNA Conc. 260/280 260/230 Yield 1 58.6 ng/µl 1.86 1.81 2.1 µg 2 68.1 ng/µl 1.87 1.81 2.4 µg 3 74.2 ng/µl 1.87 1.83 2.6 µg Table 2. RNA was purified using the TriRNA Pure Kit. RNA from 200 µl of whole human blood (3 donors) was initally homogenized using GENEzol™ Reagent. RNA was then purified using a spin column procedure. 10 µl from each 35 µl eluate of purified RNA was analyzed by electrophoresis on a 0.8% agarose gel. M = Geneaid 1 Kb DNA Ladder