Tissue Total RNA Mini Kit (RT050, RT100, RT300)

The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. The Total RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes. 

Advantages (Cat. # RT050, RT100, RT300)

Total RNA Mini Kit (Tissue) Functional Test Data 

Figure 1. Total RNA was extracted from 10 mg of various mouse tissue using the Total RNA Mini Kit (Tissue). 5 μl aliquots of each sample was analyzed by electrophoresis on a 1% agarose gel.   1. Brain, 2. Lung, 3. Liver, 4. Spleen, 5. Kidney

Total RNA Extraction Kits Specifications

Specifications	Total RNA Mini Kit Blood Cultured Cell	Total RNA Mini Kit Tissue	Total RNA Mini Kit Plant	Presto™ Mini RNA Bacteria Kit	Presto™ Mini RNA Yeast Kit	miRNA Isolation Kit	GENEzol™ TriRNA Pure Kit
Cat. #	RB050 RB100 RB300	RT050 RT100 RT300	RP050 RP100 RP300	RBB050 RBB100 RBB300	RBY050 RBY100 RBY300	RMI050	GZX050 GZX100 GZX200
Format	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Tri Reagent Spin Column
Binding Capacity	50 µg	50 µg	50 µg	50 µg	50 µg	50 µg	50 µg
Sample	blood, cells	animal tissue	plant tissue	bacterial cells	yeast cells	animal tissue, cells	blood,cells, tissue, body fluids, bacteria
Micropestle	N/A	Yes	N/A	N/A	N/A	Yes	N/A
Filter Column	N/A	Yes	Yes	N/A	N/A	N/A	N/A
Enzyme	N/A	N/A	N/A	Lysozyme	N/A	N/A	N/A
Typical Yield	2-3 µg from 300 µl blood, 25-30 µg from 106 cells	5-30 µg	5-30 µg	40-50 µg from 1x109 bacterial cells	15-20 µg from 5x107 yeast cells	5-30 µg	5-30 µg
Elution Volume	50 µl	50 µl	50 µl	50 µl	50 µl	50 µl	25-50 µl
Time	<20 min.	<15 min.	<20 min.	<20 min.	<20 min.	<30 min.	<15 min.

Related RNA Extraction Products

Product Name	RXNS	Catalogue Number
Total RNA Mini Kit (Blood/Cultured Cell)	50, 100, 300	RB050/100/300, RBD050/100/300
Total RNA Mini Kit (Tissue)	50, 100, 300	RT050/100/300, RTD050/100/300
Total RNA Mini Kit (Plant)	50, 100, 300	RP050/100/300, RPD050/100/300
Presto™ Mini RNA Bacteria Kit	50, 100, 300	RBB050/100/300, RBBD050/100/300
Presto™ Mini RNA Yeast Kit	50, 100, 300	RBY050/100/300, RBYD050/100/300
miRNA Isolation Kit	50	RMI050
GENEzol™ TriRNA Pure Kit	50, 100, 200	GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit	4 x 96, 10 x 96	96GZX04/10