Blood/Cell Total RNA Mini Kit (RB050, RB100, RB300)

The RNA Kit (Blood/Cultured Cell) is a RNA Isolation Kit designed for total RNA purification from fresh whole human blood and cultured cells. This RNA Kit will purify RNA from blood within 20 minutes using an efficient RNA miniprep system. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column and once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water and ready for use in various downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes. 

Advantages (Cat. # RB050, RB100, RB300)

Total RNA Mini Kit (Blood/Cultured Cell) Functional Test Data

	Figure 1. Total RNA from 1×106 293T cells was extracted using the Total RNA Mini Kit (Blood/Cultured Cell) and the equivalent competitors kit. The purified RNA was analyzed by electrophoresis on a 1% agarose gel. Lane 1, 2 = Geneaid, M = Geneaid 1 Kb DNA Ladder Lane 3, 4 = Competitor Geneaid Competitor Yield (μg) 260/280 260/230 Yield (μg) 260/280 260/230 28.70 1.96 2.32 20.50 1.85 2.31 29.90 1.99 2.02 28.90 1.84 2.32
	Figure 2. Total RNA from 300 µl of whole human blood was extracted using the Total RNA Mini Kit (Blood/Cultured Cell) and the equivalent competitors kit. The purified RNA was analyzed by electrophoresis on a 1% agarose gel. Lane 1, 2 = Geneaid M = Geneaid 1 Kb DNA Ladder, Lane 3, 4 = Competitor Geneaid Competitor Yield (μg) 260/280 260/230 Yield (μg) 260/280 260/230 2.02 1.85 2.15 1.77 1.78 1.42 2.13 1.90 2.35 1.80 1.76 1.19
Figure 2. Total RNA was purified from 101-103 HeLa cells using the Total RNA Mini Kit Blood/Cultured Cell (2 replications of each cell number). The purified RNA was eluted with 25 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA (2/5) using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 8 µl of synthesized cDNA (2/5) as template, primers (designed to amplify human ACTB gene), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that RNA can be successfully extracted and detected from as low as 101 HeLa cells. Legend 103 HeLa Cells, 102 HeLa Cells, 101 HeLa Cells

Total RNA Extraction Kits Specifications

Specifications	Total RNA Mini Kit Blood Cultured Cell	Total RNA Mini Kit Tissue	Total RNA Mini Kit Plant	Presto™ Mini RNA Bacteria Kit	Presto™ Mini RNA Yeast Kit	miRNA Isolation Kit	GENEzol™ TriRNA Pure Kit
Cat. #	RB050 RB100 RB300	RT050 RT100 RT300	RP050 RP100 RP300	RBB050 RBB100 RBB300	RBY050 RBY100 RBY300	RMI050	GZX050 GZX100 GZX200
Format	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Tri Reagent Spin Column
Binding Capacity	50 µg	50 µg	50 µg	50 µg	50 µg	50 µg	50 µg
Sample	blood, cells	animal tissue	plant tissue	bacterial cells	yeast cells	animal tissue, cells	blood,cells, tissue, body fluids, bacteria
Micropestle	N/A	Yes	N/A	N/A	N/A	Yes	N/A
Filter Column	N/A	Yes	Yes	N/A	N/A	N/A	N/A
Enzyme	N/A	N/A	N/A	Lysozyme	N/A	N/A	N/A
Typical Yield	2-3 µg from 300 µl blood, 25-30 µg from 106 cells	5-30 µg	5-30 µg	40-50 µg from 1x109 bacterial cells	15-20 µg from 5x107 yeast cells	5-30 µg	5-30 µg
Elution Volume	50 µl	50 µl	50 µl	50 µl	50 µl	50 µl	25-50 µl
Time	<20 min.	<15 min.	<20 min.	<20 min.	<20 min.	<30 min.	<15 min.

Related RNA Extraction Products

Product Name	RXNS	Catalogue Number
Total RNA Mini Kit (Blood/Cultured Cell)	50, 100, 300	RB050/100/300, RBD050/100/300
Total RNA Mini Kit (Tissue)	50, 100, 300	RT050/100/300, RTD050/100/300
Total RNA Mini Kit (Plant)	50, 100, 300	RP050/100/300, RPD050/100/300
Presto™ Mini RNA Bacteria Kit	50, 100, 300	RBB050/100/300, RBBD050/100/300
Presto™ Mini RNA Yeast Kit	50, 100, 300	RBY050/100/300, RBYD050/100/300
miRNA Isolation Kit	50	RMI050
GENEzol™ TriRNA Pure Kit	50, 100, 200	GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit	4 x 96, 10 x 96	96GZX04/10