Small DNA Fragments Kit (DF101, DF301)

The Small DNA Fragments Extraction Kit is a Gel Extraction Kit and PCR Cleanup Kit in 1 convenient system optimized to recover or concentrate 40-200 bp DNA fragments from agarose gel, PCR, or other enzymatic reactions. For processing 70 bp-10 kb DNA fragments, the Gel/PCR DNA Fragments Extraction Kit is also available. For processing 10-50 kb DNA fragments, the Large DNA Fragments Extraction Kit is also available. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are up to 90% for Gel Extraction and up to 95% for PCR Cleanup. The eluted DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.  

Advantages (Cat. # DF101, DF301)

• Purify small DNA in as little as 10 minutes!
• Up to 95% recovery of small DNA fragments 
• Optimized for 40 bp–200 bp DNA fragment recovery
• Sample Size: up to 300 mg (agarose gel slice), up to 100 μl (PCR products)
• Spin Columns: glass fiber membrane optimized for both gel extraction and PCR cleanup of small DNA fragments
• Elution Volume: 20-50 µl
• Storage: dry at room temperature (15-25ºC)

• SD Buffer
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• SD Columns
• 2 ml Collection Tubes

Quality Control

The quality of the Small DNA Fragments Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions and analyzed by electrophoresis.

Small DNA Fragments Extraction Kit Interactive Protocol

Small DNA Fragments Extraction Kit Functional Test Data

Figure 1. A 60 bp DNA fragment from Escherichia coli 16S ribosomal DNA (1 µg) was extracted from a PCR product using the Small DNA Fragments Extraction Kit. The recovered DNA concentration was determined by spectrophotometer and analyzed by electrophoresis on a 1.5% agarose gel. Lane 1: Unpurified 60 bp DNA fragment (1 µg), Lane 2: Purified 60 bp DNA fragment (0.71 µg), M = Geneaid 100 bp DNA Ladder DNA Conc. 260/280 Elution Volume Total yield Recovery 14.20 μg/ml 1.73 50 µl 0.71 μg 71%

Figure 2. A 60 bp DNA fragment from Escherichia coli 16S ribosomal DNA (1 µg) was extracted from gel using the Small DNA Fragments Extraction Kit. The recovered DNA concentration was determined by spectrophotometer and analyzed by electrophoresis on a 1.5% agarose gel. Lane 1: Unpurified 60 bp DNA fragment (1 µg), Lane 2: Purified 60 bp DNA fragment (0.60 µg), M = Geneaid 100 bp DNA Ladder DNA Conc. 260/280 Elution Volume Total yield Recovery 12.00 μg/ml 1.63 50 µl 0.60 μg 60%