The GenepHlow™ Gel Extraction Kit was designed to recover or concentrate DNA fragments from agarose gel. This gel extraction kit includes QG Buffer premixed with a pH indicator to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. 

Advantages (Cat. # DFG100, DFG300)

GenepHlow™ Gel Extraction Kit Functional Test Data                

			Figure 1. Gel slice DNA fragments ranging from 250 bp-10 kb were extracted using the GenepHlow™ Gel Extraction Kit (lane 1,2,3). The purified DNA from a 50 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder (control, total DNA = 1100 ng) Test DNA Conc. 260/280 Yield Recovery 1 18.2 ng/µl 1.84 910 ng 82% 2 18.7 ng/µl 1.82 935 ng 85% 3 19.2 ng/µl 1.82 960 ng 87.3%
Optimal pH        pH Too High		Figure 2. A pH indicator is premixed with the binding buffer to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow.

Gel Extraction and PCR Cleanup Kits Specifications

Specifications	GenepHlow™ Gel Extraction Kit	GenepHlow™ PCR Cleanup Kit	GenepHlow™ Gel/PCR Kit	GenepHlow™ DNA Cleanup Maxi Kit	Presto™ 96 Well Gel Extraction Kit	Small DNA Fragments Extraction Kit	Large DNA Fragments Extraction Kit
Cat. #	DFG100 DFG300	DFC100 DFC300	DFH100 DFH300	DFM010 DFM025	96DFG04 96DFG10	DF101 DF301	DFL100 DFL300
Format	Spin Column	Spin Column	Spin Column	Maxi Spin Column	96 Well Plate	Spin Column	Silica Matrix
Binding Capacity	10 µg	10 µg	10 µg	200 µg	10 µg	10 µg	5 µg
Sample	Agarose Gel Slice	PCR Product	Agarose Gel Slice/PCR Product	Agarose Gel Slice/Restriction Enzyme Digest Product	Agarose Gel Slice	Agarose Gel Slice/PCR Product	Agarose Gel Slice/PCR Product
Fragment Size	70 bp-20 kb	70 bp-20 kb	70 bp-20 kb	100 bp-10 kb	70 bp-20 kb	40-200 bp	100 bp-50 kb
pH Indicator	Yes	Yes	Yes	Yes	N/A	N/A	Yes
Recovery	Up to 90%	Up to 95%	Up to 95%	Up to 80%	Up to 80%	Up to 95%	Up to 95% (<10 kb) Up to 80% (10-50 kb)
Elution Volume	20-50 µl	20-50 µl	20-50 µl	500-1000 µl	60-80 µl	20-50 µl	10-20 µl
Operation Time	20 minutes	10 minutes	<20 minutes	<25 minutes	45 minutes	<20 minutes	<20 minutes

Related Gel Extraction and PCR Cleanup Products

Product Name	RXNS	Catalogue Number
GenepHlow™ Gel Extraction Kit	100, 300	DFG100/300
GenepHlow™ PCR Cleanup Kit	100, 300	DFC100/300
GenepHlow™ Gel/PCR Kit	100, 300	DFH100/300
GenepHlow™ DNA Cleanup Maxi Kit	10, 25	DFM010/25
Presto™ 96 Well PCR Cleanup Kit	4 x 96, 10 x 96	96DFH04/10
Small DNA Fragments Extraction Kit	100, 300	DF101/301
Large DNA Fragments Extraction Kit	100, 300	DFL100/300
Gel/PCR DNA Fragments Extraction Kit	100, 300	DF100/300
Presto™ 96 Well Gel Extraction Kit	4 x 96, 10 x 96	96DFG04/10
GenepHlow™ DNA Cleanup Midi Kit	100, 300	DFI100/300