Plant DNA Isolation Kit (GR100, GR200)

GENEzol™ DNA Reagent Plant provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP without phenol extraction. 

Advantages (Cat. # GR100, GR200)

• Extract high molecular weight genomic DNA from a variety of plant species
• Sample Volume: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
• A cost effective, scalable solution
• Format: simple and gentle CTAB and chloroform based DNA precipitation method
• Storage: dry at room temperature (15-25ºC) for up to 2 years, RNase should be stored at 4ºC for extended periods

• GENEzol™ DNA Reagent Plant
• RNase A (50 mg/ml)



Figure 1. Genomic DNA (approximately 30 kb) was extracted using GENEzol™ DNA Reagent Plant. 50 mg of fresh Arabidopsis leaves were initially ground in GENEzol™ DNA Reagent Plant. A 15 µl aliquot of extracted genomic DNA from a 50 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder Test DNA Conc. 260/280 260/230 Yield 1 380.7 µg/ml 1.97 1.88 19.04 µg 2 316.9 µg/ml 1.96 1.89 15.85 µg

Figure 1. Genomic DNA (approximately 30 kb) was extracted using GENEzol™ DNA Reagent Plant. 50 mg of fresh rose leaves were initially ground in GENEzol™ DNA Reagent Plant. A 5 µl aliquot of extracted genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder Test DNA Conc. 260/280 260/230 Yield 1 109.5 µg/ml 1.82 1.71 11.0 µg 2 106.0 µg/ml 1.82 1.73 10.6 µg 3 99.4 µg/ml 1.81 1.71 9.9 µg