• Isolate high molecular weight DNA for archiving or sensitive downstream applications
• High yield, High quality DNA (A260/A280 = 1.8-2.0)
• Sample: 0.5-1.5 x 109 cultured cells
• Easily scalable to satisfy larger cultured cell sample volumes
• Minimal hands on time
• Convenient, simple and gentle reagent DNA precipitation method
• Storage: dry at room temperature (15-25ºC) for up to 2 years, RNase A should be stored at -20ºC for extended periods

Applications

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR 

Components

• Cell Lysis Buffer
• Protein Removal Buffer 
• DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
• RNase A (10 mg/ml) - not included with GEC1.5K

Quality Control

The quality of the Geneaid™ DNA Isolation Kit (Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is isolated from 0.5-1.5 x 109 cultured cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.