상품 정보
상품 상세설명
The Midiprep Spin Column Plasmid Kit was designed for plasmid and cosmid DNA purification from 50-100 ml of cultured bacterial cells using a plasmid midiprep spin column system. This plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water. Typical yields are 200-350 μg for high-copy number plasmid or 30-100 μg for low-copy number plasmid from 50 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 35 minutes.
Advantages (Cat. # PA025)
- Purify plasmid DNA from large volume samples within 35 minutes!
- Midi spin column format
- High Yield: up to 400 μg of plasmid DNA
- Large Sample Volume: 50-100 ml of cultured bacterial cells
- Glass fiber membrane optimized for plasmid DNA purification from large volume bacteria samples
- Elution Volume: 1 ml
- Storage: dry at room temperature (15-25ºC)
Applications
Restriction Enzyme Digestion, Library Screening, Ligation, PCR, Transformation and Sequencing Reactions
Components
- PD1, PD2, PD3 Buffers
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- RNase A
- PA Columns
Quality Control
The quality of the Midiprep Spin Column Plasmid Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 50 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the High-Speed Plasmid Advance Kit. Following the purification process, a yield of more than 200 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and checked by electrophoresis.
 | Figure 1. Plasmid DNA was extracted using the Midiprep Spin Column Plasmid Kit. 5 μl aliquots of purified supercoiled plasmid DNA [100 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript] from a 1 ml eluate were analyzed by electrophoresis on a 1% agarose gel. M = Geneaid™ 1 Kb DNA Ladder Lane 1-2: pBluescript
|
| Product Name | RXNS | Catalogue Number |
| Presto™ Endotoxin Free Mini Plasmid Kit | 100 | PEH100 |
100, 300 | PDH100/300 | |
100, 300 | PD100/300 | |
100, 300 | PDL100/300 | |
Midiprep Spin Column Plasmid Kit | 25 | PA025 |
25 | PIF025 | |
25 | PIFE25 | |
| Geneaid™ Midi Plasmid Kit | 25 | PI025 |
| Geneaid™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIE25 |
| Presto™ Plasmid DNA Concentration Kit | 25, 50, 100 | PC0250/500/1000 |
| Geneaid™ Maxi Plasmid Kit | 10, 25 | PM010/25 |
| Geneaid™ Maxi Plasmid Kit (Endotoxin Free) | 10, 25 | PME10/25 |
| Presto™ 96 Well Plasmid Kit | 4 x 96, 10 x 96 | 96PDC04/10 96PDV04/10 |
상품문의
등록된 상품문의
상품문의가 없습니다.
반품/교환정보
셀젠바이오샵에서는 다음과 같은 기간 및 내용으로 상품에 대하여 교환, 반품, 환불을 보장하고 있으며, 상품의 반환에 의한추가비용을 고객에게 부담시지키 않습니다. (단, 고객 변심 또는 주문 반복으로 인한 경우의 반환비용은 고객님이 부담하셔야
합니다.)
::: 교환 및 반품이 가능한 경우:::
단, 상품을 개봉하여 상품가치가 상실된 경우에는 교환/반품이 불가능합니다.
:::교환 및 반품이 불가능한 경우:::
주문 취소 및 반품으로 환불을 요청하실 경우에는 E-mail(celgen-bio@celgen-bio.com)이나 고객만족센터 (042-824-9026)을
통해 요청하시면 친절하게 처리해 드리겠습니다.
주문 취소 후 반품 가능 여부를 확인한 다음 3일 이내에 결제 금액을 환불해 드리겠습니다.