Maxi Plasmid DNA Kit (PM010, PM025)

The Geneaid™ Maxiprep Plasmid Kit was designed for plasmid DNA purification using pre-packed anion-exchange resin maxiprep plasmid columns to purify plasmid DNA from 200-1600 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. This plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the plasmid maxi column and the contaminants are removed with PMC Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 100 minutes without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for Transfection, Sequencing Reactions, Ligation, PCR, and In-vitro Transcription, Microinjection, Restriction Enzyme Digestion and Gene Gun. 

Advantages (Cat. # PM010, PM025)

• High Yield: 1.0 mg of pure transfection grade plasmid DNA from 300 ml cultured bacterial cells
• Large Sample Volume: 200-1600 ml of cultured bacterial cells
• Format: plasmid maxi anion-exchange resin columns
• Elution Volume: 500 µl - 2 ml
• Operation time: within 100 minutes
• Storage: dry at room temperature (15-25ºC)

Applications

Transfection, Sequencing, Ligation, PCR, In-vitro Transcription Microinjection, Restriction Enzyme Digestion, Gene Gun

Components

• PM1, PM2, PM3 Buffer
• TrueBlue Lysis Buffer
• PEQ Buffer
• PMC Buffer
• PEL Buffer
• RNase A
• Plasmid Maxi Columns

Quality Control

The quality of the Geneaid™ Plasmid Maxi Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 300 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Maxi Plasmid Kit. Following the purification process, a yield of more than 900 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is analyzed by electrophoresis.

Plasmid Maxi Kit Funtional Test Data

Figure 1. Plasmid DNA was extracted using the Geneaid™ Maxi Maxi Kit. The purified supercoiled plasmid DNA [400 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (A600 > 2 U/ml, OD600 = 4.0)], was analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder Sample DNA Conc. 260/280 260/230 Total Yield Test 1 1012.2 µg/ml 1.87 2.25 1.0 mg Test 2 1015.1 µg/ml 1.87 2.29 1.0 mg

Plasmid DNA Midi and Maxi Kits Specifications

Specifications	Presto™ Midi Plasmid Kit	Presto™ Midi Plasmid Kit Endotoxin Free	Geneaid™ Midi Plasmid Kit	Geneaid™ Midi Plasmid Kit Endotoxin Free	Geneaid™ Maxi Plasmid Kit	Geneaid™ Maxi Plasmid Kit Endotoxin Free
Cat. #	PIF025	PIFE25	PI025	PIE25	PM010/25	PME10/25
Format	Anion-Exchange Gravity Flow	Anion- Exchange Gravity Flow	Anion-Exchange Gravity Flow	Anion- Exchange Gravity Flow	Anion-Exchange Gravity Flow	Anion- Exchange Gravity Flow
Binding Capacity	350 µg	350 µg	350 µg	350 µg	1.0 mg	1.0 mg
Filter Column	Yes	Yes	N/A	N/A	N/A	N/A
Endotoxin Level	<0.15 EU/µg	<0.10 EU/µg	<0.15 EU/µg	<0.10 EU/µg	<0.15 EU/µg	<0.10 EU/µg
Culture Input	50-150 ml	50-150 ml	50-300 ml	50-300 ml	200-1600 ml	200-1600 ml
Plasmid Size	1-20 kb	1-20 kb	1-20 kb	1-20 kb	1-20 kb	1-20 kb
Typical Yield	350 µg	350 µg	350 µg	350 µg	1.0 mg	1.0 mg
Elution Volume	500 µl-2 ml	500 µl-2 ml	500 µl-2 ml	500 µl-2 ml	500 µl-2 ml	500 µl-2 ml
Operation Time	<80 minutes	<80 minutes	<80 minutes	<80 minutes	<100 minutes	<100 minutes

Related Plasmid DNA Purification Products

Product Name	RXNS	Catalogue Number
Presto™ Endotoxin Free Mini Plasmid Kit	100	PEH100
Presto™ Mini Plasmid Kit	100, 300	PDH100/300
High-Speed Plasmid Mini Kit	100, 300	PD100/300
Large Plasmid DNA Extraction Kit	100, 300	PDL100/300
High-Speed Plasmid Advance Kit (50-100 ml)	25	PA025
Presto™ Midi Plasmid Kit	25	PIF025
Presto™ Midi Plasmid Kit (Endotoxin Free)	25	PIFE25
Geneaid™ Midi Plasmid Kit	25	PI025
Geneaid™ Midi Plasmid Kit (Endotoxin Free)	25	PIE25
Presto™ Plasmid DNA Concentration Kit	25, 50, 100	PC0250/500/1000
Geneaid™ Maxi Plasmid Kit	10, 25	PM010/25
Geneaid™ Maxi Plasmid Kit (Endotoxin Free)	10, 25	PME10/25
Presto™ 96 Well Plasmid Kit	4 x 96, 10 x 96	96PDC04/10 96PDV04/10