상품 정보
상품 기본설명
상품 상세설명
The Geneaid™ Midi Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50-300 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA is bound to the column and contaminants are efficiently removed. The purified plasmid DNA is eluted then precipitated with isopropanol for desalting. The entire procedure can be completed without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.
Advantages (Cat. # PI025)
- High Yield: 350 µg of pure transfection grade plasmid DNA from 100 ml of cultured bacterial cells
- Sample Volume: 50-300 ml of cultured bacterial cells
- Format: plasmid midi anion-exchange resin column
- Elution Volume: 500 µl - 2 ml
- Operation time: within 80 minutes (only 50 minutes when combined with the Presto™ Plasmid Concentrator)
- Storage: dry at room temperature (15-25ºC)
Applications
Transfection, Sequencing, Ligation, PCR, In-vitro Transcription, Microinjection, Restriction Enzyme Digestion, Gene Gun
Components
- PM1, PM2, PM3 Buffer
- TrueBlue Lysis Buffer
- PEQ Buffer
- PMC Buffer
- PEL Buffer
- RNase A
- Plasmid Midi Columns
Quality Control
The quality of the Geneaid™ Midi Plasmid Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 100 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Midi Plasmid Kit. Following the purification process, a yield of more than 300 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
Plasmid Midi Kit Functional Test Data
 | Figure 1. Plasmid DNA from a 100 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (OD600 = 3.65) was purified using the Geneaid™ Midi Plasmid Kit. The purified supercoiled plasmid DNA was used in EcoRI digestion. Plasmid DNA yield was determined by spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
|
| Specifications | ||||||
| Cat. # | PIF025 | PIFE25 | PI025 | PIE25 | PM010/25 | PME10/25 |
Format | Anion-Exchange Gravity Flow | Anion- Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion- Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion- Exchange Gravity Flow |
| Binding Capacity | 350 µg | 350 µg | 350 µg | 350 µg | 1.0 mg | 1.0 mg |
| Filter Column | Yes | Yes | N/A | N/A | N/A | N/A |
| Endotoxin Level | <0.15 EU/µg | <0.10 EU/µg | <0.15 EU/µg | <0.10 EU/µg | <0.15 EU/µg | <0.10 EU/µg |
| Culture Input | 50-150 ml | 50-150 ml | 50-300 ml | 50-300 ml | 200-1600 ml | 200-1600 ml |
| Plasmid Size | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb |
| Typical Yield | 350 µg | 350 µg | 350 µg | 350 µg | 1.0 mg | 1.0 mg |
| Elution Volume | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml |
| Operation Time | <80 minutes | <80 minutes | <80 minutes | <80 minutes | <100 minutes | <100 minutes |
| Product Name | RXNS | Catalogue Number |
| Presto™ Endotoxin Free Mini Plasmid Kit | 100 | PEH100 |
100, 300 | PDH100/300 | |
100, 300 | PD100/300 | |
100, 300 | PDL100/300 | |
25 | PA025 | |
25 | PIF025 | |
25 | PIFE25 | |
| Geneaid™ Midi Plasmid Kit | 25 | PI025 |
| Geneaid™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIE25 |
| Presto™ Plasmid DNA Concentration Kit | 25, 50, 100 | PC0250/500/1000 |
| Geneaid™ Maxi Plasmid Kit | 10, 25 | PM010/25 |
| Geneaid™ Maxi Plasmid Kit (Endotoxin Free) | 10, 25 | PME10/25 |
| Presto™ 96 Well Plasmid Kit | 4 x 96, 10 x 96 | 96PDC04/10 96PDV04/10 |
상품문의
등록된 상품문의
상품문의가 없습니다.
반품/교환정보
셀젠바이오샵에서는 다음과 같은 기간 및 내용으로 상품에 대하여 교환, 반품, 환불을 보장하고 있으며, 상품의 반환에 의한추가비용을 고객에게 부담시지키 않습니다. (단, 고객 변심 또는 주문 반복으로 인한 경우의 반환비용은 고객님이 부담하셔야
합니다.)
::: 교환 및 반품이 가능한 경우:::
단, 상품을 개봉하여 상품가치가 상실된 경우에는 교환/반품이 불가능합니다.
:::교환 및 반품이 불가능한 경우:::
주문 취소 및 반품으로 환불을 요청하실 경우에는 E-mail(celgen-bio@celgen-bio.com)이나 고객만족센터 (042-824-9026)을
통해 요청하시면 친절하게 처리해 드리겠습니다.
주문 취소 후 반품 가능 여부를 확인한 다음 3일 이내에 결제 금액을 환불해 드리겠습니다.