Exosomes are small membrane vesicles (30–150 nm) of endocytic origin, which are shed by all cell types under normal- and patho-physiological conditions. Exosomes have pleiotropic physiological and pathological functions and play significant roles in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
Exosomes have received significant attention for their roles in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed for exosome isolation such as differential ultracentrifugation, density gradient ultracentrifugation, ultracentrifugation, chromatography, magnetic bead immunoassay, polypolymer precipitation method, etc.
CUSABIO Exosome Isolation Kit purifies exosome by affinity purification. High Purity exosomes and other Evs (Extracellular vesicles) from cell culture medium and body fluid (high yield by normal microfiltration) can be easily obtained by this method.
Exosomes isolated by this product are suitable for the following downstream:
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